How to use the opentrons.labware function in opentrons

sample_num: int=96,
        old_media_volume: float=200,
        trypsin_volume: float=200,
        trypsin_wait_time: int=5,
        wash_volume: float=200,
        mix_times: int=3,
        new_media_volume: float=200):

    global pipette_m, pipette_s

    # labware setup
    old_plates = [labware.load(cell_container, '1')]
    new_plates = [labware.load(cell_container, '2')]
    pcr_plate = labware.load('96-PCR-tall', '3')
    trough = labware.load('trough-12row', '4')
    tuberack_15ml = labware.load('opentrons-tuberack-15ml', '5')

    # reagent setup
    trypsin = tuberack_15ml.wells('A1')
    PBS = trough.wells('A1')
    media = trough.wells('A4')
    liquid_trash = trough.wells('A9', to='A12')

    s_name = single_pipette_model.split('-')[0]
    m_name = multi_pipette_model.split('-')[0]

    # if using 24-well plate, only single channel pipette is loaded
    if cell_container == '24-well-plate':
        s_slots = ['6', '7', '8', '9', '10', '11']
    else:
        s_slots = ['10', '11']
        m_slots = ['6', '7', '8', '9']

'author': 'Nick ',
    'source': 'Custom Protocol Request'
    }

# module
module = modules.load('tempdeck', '2')
strips = labware.load(
    'opentrons-aluminum-block-PCR-strips-200ul',
    '2',
    share=True
    )
module.set_temperature(4)
module.wait_for_temp()

# labware
tuberack = labware.load('tube-rack-2ml', '1')
tiprack = labware.load('tiprack-10ul', '4')

# pipettes
p10 = instruments.P10_Single(
    mount='right',
    tip_racks=[tiprack]
)

# reagent and PCR strip setup
h2o = tuberack.wells('A1')
primer1 = tuberack.wells('A2')
primer2 = tuberack.wells('A3')
dreamtaq_buffer = tuberack.wells('A4')
dNTP = tuberack.wells('A5')
dreamtaq_polymerase = tuberack.wells('A6')
mastermix = tuberack.wells('B1')

from opentrons import labware, instruments, modules, robot

# labware setup
mag_deck = modules.load('magdeck', '1')
mag_plate = labware.load('96-deep-well', '1', share=True)
plate = labware.load('96-deep-well', '2')
trough = labware.load('trough-12row', '3')
liquid_trash = labware.load('point', '4')
flat_plate = labware.load('96-flat', '5')

# reagent setup
RNase_A = trough.wells('A1')
solution_II = trough.wells('A2')
N3_buffer = trough.wells('A3')
LC_beads = trough.wells('A4')
ETR_bind_buffer = trough.wells('A5')
Mag_Bind_RQ = trough.wells('A6')
ETR_wash_buffer = trough.wells('A7')
VHB_buffer = trough.wells('A8')
SPM_wash_buffer = trough.wells('A9')
elution_buffer = trough.wells('A10')

# create labware
plate_name = 'Falcon-96-round'
if plate_name not in labware.list():
    labware.create(
        plate_name,
        grid=(12, 8),
        spacing=(9, 9),
        diameter=6.35,
        depth=10.59,
        volume=320
    )

# load labware and modules
tempdeck = modules.load('tempdeck', '1')
plate2 = labware.load(plate_name, '1', share=True)
plate1 = labware.load(plate_name, '2', 'plate 1')
plate3 = labware.load(plate_name, '3', 'plate 3')
trough = labware.load('trough-12row', '4')
tuberack = labware.load('opentrons-tuberack-2ml-eppendorf', '5')
tips = [labware.load('tiprack-10ul', slot) for slot in ['6', '7']]

# pipettes
p10 = instruments.P10_Single(mount='right', tip_racks=tips)

# setup wells
plate1_wells = [well for row in plate1.rows() for well in row]
plate2_wells = [well for row in plate2.rows() for well in row]
plate3_wells = [well for row in plate3.rows() for well in row]
tube = tuberack.wells('A1')
solution = trough.wells('A1')

sources = []
    volumes = []
    dests = []
    mixes = []
    plates = {}
    for line in info_list[1:]:
        if line[0] not in reagent_list.keys():
            raise Exception('Source container in slot ' + line[0] +
                            ' does not exist.')
        else:
            source = reagent_list[line[0]].wells(line[1])

        volume = float(line[2])

        if line[3] not in plates.keys():
            plates[line[3]] = labware.load(
                dest_plate, line[3], share=True)

        if not line[5].lower().strip() == 'yes':
            mixes.append("")
        else:
                mixes.append(True)

        dest = plates[line[3]].wells(line[4])
        sources.append(source)
        volumes.append(volume)
        dests.append(dest)

    return sources, volumes, dests, mixes

from opentrons import labware, instruments

metadata = {
    'protocolName': 'RBC Transfer',
    'author': 'Alise ',
    'source': 'Custom Protocol Request'
    }

plate_name = 'omegaquant-96-well-plate'
if plate_name not in labware.list():
    labware.create(
        plate_name,
        grid=(12, 8),
        spacing=(9, 9),
        diameter=9,
        depth=30)

trough_name = 'glass-trough'
if trough_name not in labware.list():
    labware.create(
        trough_name,
        grid=(1, 1),
        spacing=(0, 0),
        diameter=66,
        depth=25)

from opentrons import labware, instruments

vol = 100  # in ul, can change

# tip rack for p300 pipette
tip300_rack = labware.load('tiprack-200ul', '1')

# 96 well plate
plate = labware.load('96-PCR-flat', '2')

cells = labware.load('tube-rack-2ml', '3')

# p100 (10 - 100 uL) (single)
p300 = instruments.P300_Single(
    mount='right',
    tip_racks=[tip300_rack])

p300.pick_up_tip()

# dilution steps
for col in cells.cols():
    p300.transfer(vol, col[3:1:-1], col[2:0:-1])

# biofilm setup steps
for col in range(0, 6):

def run_custom_protocol(pipette_type: StringSelection(
        'p10-single', 'p50-multi')='p10-single',
        mm_volume: int=10,
        titrator_volume: int=10,
        dilution_volume: int=10):

    experiment_plate = labware.load('384-plate', '3')

    well_type = False  # Variable showing Well object not list of wells
    if pipette_type == 'p10-single':
        # Use tuberack if p10 single pipette
        tuberack = labware.load('opentrons-tuberack-2ml-eppendorf', '1')
        master_mix = tuberack.wells('A1')
        ab_titrator = tuberack.wells('B1')
        well_type = True
        tiprack = labware.load('tiprack-10ul', '2')
        pipette = instruments.P10_Single(mount='right', tip_racks=[tiprack])
    elif pipette_type == 'p50-multi':
        # Use 96 flat if multichannel
        reagent_plate = labware.load('96-flat', '1')
        master_mix = reagent_plate.columns('1')
        ab_titrator = reagent_plate.columns('2')
        tiprack = labware.load('opentrons-tiprack-300ul', '2')

from opentrons import labware, instruments, robot

"""
Tagment Genomic DNA
Amplify Libraries
"""
# labware setup
gDNA_plate = labware.load('96-PCR-flat', '1')
out_plate = labware.load('96-PCR-flat', '2')
# out_plate = labware.load('biorad-hardshell-96-PCR', '2')
tuberack = labware.load('tube-rack-2ml', '5')

# reagent setup
atm = tuberack.wells('A1')  # Amplicon Tagment Mix
td = tuberack.wells('B1')  # Tagment DNA Buffer
nt = tuberack.wells('C1')  # Neutralize Tagment Buffer
npm = tuberack.wells('D1')  # Nextera PCR Master Mix
index_7 = tuberack.wells('A2', to='D4')  # Index 1 (i7) adapters
index_5 = tuberack.wells('A5', to='D6')  # Index 2 (i5) adapters

tipracks50 = [labware.load('tiprack-200ul', slot) for slot in ['3', '4']]
tipracks10 = [labware.load('tiprack-10ul', slot) for slot in ['6', '7']]

# pipette setup
p50 = instruments.P50_Single(

from opentrons import labware, instruments, robot, modules
from otcustomizers import StringSelection

metadata = {
    'protocolName': 'BioFluid Mix and Transfer - Part 1/2',
    'author': 'Chaz ',
    'source': 'Custom Protocol Request'
}

# custom labware creation
cryo_container = 'custom_agilent_cryovial_container'
if cryo_container not in labware.list():
    labware.create(
        cryo_container,
        grid=(5, 10),
        spacing=(18.3, 18.3),
        diameter=12,
        depth=48,
        volume=6000
    )

centr_container = 'custom_centrifuge_tube_container'
if centr_container not in labware.list():
    labware.create(
        centr_container,
        grid=(8, 12),
        spacing=(9, 9),
        diameter=5.5,