How to use the comparem.seq_io.SeqIO function in comparem

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github dparks1134 / CompareM / comparem / prodigal.py View on Github external
Fasta file for genome.
        """

        genome_id = ntpath.basename(genome_file)
        genome_id = genome_id[0:genome_id.rfind('.')]

        aa_gene_file = os.path.join(self.output_dir, genome_id + '.genes.faa')
        nt_gene_file = os.path.join(self.output_dir, genome_id + '.genes.fna')
        gff_file = os.path.join(self.output_dir, genome_id + '.gff')

        if self.called_genes:
            os.system('ln -s %s %s' % (os.path.abspath(genome_file), aa_gene_file))
        else:
            tmp_dir = tempfile.mkdtemp()

            seqIO = SeqIO()
            seqs = seqIO.read_fasta(genome_file)

            # determine number of bases
            total_bases = 0
            for seq in seqs.values():
                total_bases += len(seq)

            # call genes under different translation tables
            table_coding_density = {}
            for translation_table in [4, 11]:
                os.makedirs(os.path.join(tmp_dir, str(translation_table)))
                aa_gene_file_tmp = os.path.join(tmp_dir, str(translation_table), genome_id + '.genes.faa')
                nt_gene_file_tmp = os.path.join(tmp_dir, str(translation_table), genome_id + '.genes.fna')
                gff_file_tmp = os.path.join(tmp_dir, str(translation_table), genome_id + '.gff')

                # check if there is sufficient bases to calculate prodigal parameters